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The expression profiles of miRNAs in thymus tissues from mice of different age have been demonstrated in our previous study. At present, in vitro cell-based assays are the standard approach for screening chemicals for cytotoxicity because of their relative simplicity, sensitivity, and cost-effectiveness compared with animal studies. Age and sex differences in microRNAs expression during the process of thymus aging. The expression of miRc-5p, miRb-5p, miRb-5p, miRp, miRp, and miR showed significant age-difference in mice thymus by quantitative polymerase chain reaction. UDCA might be a good nutrient for oral cancer prevention.

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Previous evidence has indicated that the microRNAb miRb family plays important roles in the regulation of cancer cell growth, development, differentiation, and apoptosis.

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We suggest that the cell impedance measurement approach is effective and better suited to determining the cytotoxicity of nanomaterials for environmental safety screening. Chk1 protein expression should therefore be tightly controlled both during normal growth and under stress situations. ZEA has significant toxic reaction on immune cells.

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