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Estrogen plays a very important role in a variety of biological aspects. The resulting Maid protein was purified using an anti-His antibody affinity column. Upon examination of Maid protein in various zebrafish organs, we found that the highest levels of this molecule occur in the liver. The liver showed the highest Maid levels, although relatively high expression was also noted in the intestine, kidney, and testis Fig 1A. Black arrow indicates the liver, where the highest level of Maid expression was observed. We observed that Maid was localized mainly in the cytoplasm of zebrafish hepatocytes, perhaps reflecting the regenerative potential of this organ. We also studied Maid expression during fin regeneration, which is the most frequently examined zebrafish tissue regeneration model [ 15 ].

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Evidence for a Role of the Transcriptional Regulator Maid in Tumorigenesis and Aging

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C Top: HE staining of zebrafish kidney at 6 months of age.

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The intensity of the color indicates the degree of up- red or down- green regulation. Primer sequences were as follows:. This article has been cited by other articles in PMC. Relative quantification of gene expression was also performed as described in the manual, using Rpl13a and Ef1a as internal controls.

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